EXPRESSION OF A FUNCTIONAL HUMAN INSULIN-RECEPTOR FROM A CLONED CDNA IN CHINESE-HAMSTER OVARY CELLS

被引：132

作者：

EBINA, Y

EDERY, M

ELLIS, L

STANDRING, D

BEAUDOIN, J

ROTH, RA

RUTTER, WJ

机构：

[1] UNIV CALIF SAN FRANCISCO, HORMONE RES LAB, SAN FRANCISCO, CA 94143 USA

[2] STANFORD UNIV, MED CTR, SCH MED, DEPT PHARMACOL, STANFORD, CA 94305 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1985年 / 82卷 / 23期

关键词：

D O I：

10.1073/pnas.82.23.8014

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have placed human insulin receptor cDNA into a vector under the control of the simian virus 40 (SV40) early promoter and tested its function by transient expression in microinjected Xenopus oocytes and by expression in stably transformed CHO cells. The precursor and the .alpha. and .beta. subunits of the receptor were detected by immunoprecipitation in CHO cells specifically binds 125I-labeled insulin but not insulin-like growth factor I, displays insulin-stimulated autophosphorylation of the .beta. subunit, and mediates insulin-stimulated 2-deoxyglucose uptake. We conclude that the human insulin receptor is synthesized, processed normally, and functional in this heterologous cell system.

引用

页码：8014 / 8018

页数：5

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