EFFECT OF SODIUM-BUTYRATE ON GLUCOSE-TRANSPORT AND GLUCOSE-PHOSPHORYLATING ENZYMES IN RIN-M5F CELLS

被引:9
作者
FERNANDEZMEJIA, C [1 ]
DAVIDSON, MB [1 ]
机构
[1] UNIV CALIF LOS ANGELES, CEDARS SINAI MED CTR,SCH MED,DEPT MED, DIV ENDOCRINOL & METAB,B131,8700 BEVERLY BLVD, LOS ANGELES, CA 90048 USA
关键词
DIFFERENTIATION; GLUCOKINASE; GLUCOSE TRANSPORTERS; HEXOKINASE; INSULINOMA CELL LINE; SODIUM BUTYRATE;
D O I
10.1097/00006676-199309000-00010
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Sodium butyrate is widely used to differentiate insulinoma cell lines. However, sodium has been shown to decrease glucose phosphorylation in the liver and heart and decrease the expression of glucose transporter. Since these mechanisms are essential for glucose-induced insulin secretion, the ultimate function of the pancreatic beta-cell, we investigated the effect of sodium butyrate on both glucose-phosphorylating enzymes as well as glucose transport in the pancreatic cell line RIN-m5F. Treatment of RIN-m5F cells with 2.5 mM sodium butyrate for 72 h increased by twofold both hexokinase and glucokinase (GK) activities, as well as the gene expression of GK. Sodium butyrate treatment had no effect on GLUT-1 mRNA levels but increased the GLUT-2 mRNA 3.7-fold. Kinetic analysis of 2-deoxyglucose transport displayed a single curve with K(m) = 1.2 mM and V(max) = 10.9 pmol/mug protein/min in the untreated cells, values similar to the low K(m) glucose transport reported in the pancreatic beta-cells. This low K(m) transport component markedly decreased with sodium butyrate treatment, and interestingly a second component with a higher K(m) appeared, consistent with the increase in GLUT-2 mRNA. We conclude that the differentiating action of sodium butyrate involves increases in GK and GLUT-2 gene expression, which characterizes the differentiated state of the pancreatic beta-cell. However, the inhibitory effect of sodium butyrate on low K(m) glucose transport needs to be considered in the use of this compound to promote differentiation.
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页码:589 / 596
页数:8
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共 43 条
[1]  
ANDERSON JW, 1984, P SOC EXP BIOL MED, V177, P372
[2]   PANCREATIC-ISLET A2B5-REACTIVE AND 3G5-REACTIVE GANGLIOSIDES ARE MARKERS OF DIFFERENTIATION IN RAT INSULINOMA CELLS [J].
BARTHOLOMEUSZ, RK ;
CAMPBELL, IL ;
HARRISON, LC .
ENDOCRINOLOGY, 1989, 124 (06) :2680-2685
[3]   EFFECT OF SODIUM-BUTYRATE, A DIFFERENTIATING AGENT, ON CELL-SURFACE GLYCOCONJUGATES OF A HUMAN PANCREATIC-CELL LINE [J].
BLOOM, EJ ;
SIDDIQUI, B ;
HICKS, JW ;
KIM, YS .
PANCREAS, 1989, 4 (01) :59-64
[4]   INHIBITION OF GLUCOSE PHOSPHORYLATION BY FATTY-ACIDS IN THE PERFUSED RAT-HEART [J].
CHATHAM, J ;
GILBERT, HF ;
RADDA, GK .
FEBS LETTERS, 1988, 238 (02) :445-449
[5]   TRANSPLANTABLE INSULINOMA IN RAT [J].
CHICK, WL ;
WARREN, S ;
CHUTE, RN ;
LIKE, AA ;
LAURIS, V ;
KITCHEN, KC .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (02) :628-632
[6]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[7]   GLYBURIDE-STIMULATED GLUCOSE-TRANSPORT IN CULTURED MUSCLE-CELLS VIA PROTEIN KINASE-C-MEDIATED PATHWAY REQUIRING NEW-PROTEIN SYNTHESIS [J].
DAVIDSON, MB ;
MOLNAR, IG ;
FURMAN, A ;
YAMAGUCHI, D .
DIABETES, 1991, 40 (11) :1531-1538
[8]   REGULATION OF GLUCOKINASE AND PROINSULIN GENE-EXPRESSION AND INSULIN-SECRETION IN RIN-M5F CELLS BY DEXAMETHASONE, RETINOIC ACID, AND THYROID-HORMONE [J].
FERNANDEZMEJIA, C ;
DAVIDSON, MB .
ENDOCRINOLOGY, 1992, 130 (03) :1660-1668
[9]  
GARDNER SL, 1989, DIABETES RES CLIN EX, V12, P93
[10]   D-GLUCOSE TRANSPORT AND CONCENTRATION IN TUMORAL INSULIN-PRODUCING CELLS [J].
GIROIX, MH ;
SENER, A ;
MALAISSE, WJ .
AMERICAN JOURNAL OF PHYSIOLOGY, 1986, 251 (06) :C847-C851