A RAPID LUMINESCENT-PHAGE BASED MPN METHOD FOR THE ENUMERATION OF SALMONELLA-TYPHIMURIUM IN ENVIRONMENTAL-SAMPLES

被引：33

作者：

TURPIN, PE

MAYCROFT, KA

BEDFORD, J

ROWLANDS, CL

WELLINGTON, EMH

机构：

[1] UNIV WARWICK,DEPT BIOL SCI,COVENTRY CV4 7AL,W MIDLANDS,ENGLAND

[2] AMERSHAM INT PLC,AMERSHAM LABS,AMERSHAM HP7 9LL,ENGLAND

[3] SEVERN TRENT WATER PLC,BIRMINGHAM B26 3PU,ENGLAND

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1993年 / 16卷 / 01期

关键词：

D O I：

10.1111/j.1472-765X.1993.tb01364.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Materials such as soils, waters, sewage sludges and foods can contain low numbers of salmonellas. A most-probable-number (MPN) method that utilized a bioluminescent-bacteriophage is described that allowed the specific determination of as few as one Salmonella typhimurium cell/100 ml of material within 24 h. The method was developed with soil, lake water and sewage sludge inoculated with Salm. typhimurium and had an efficiency of 100% when tested against a traditional MPN method. The protocol is rapid, sensitive, inexpensive, has a low operator time compared to the traditional MPN method, allows for the repair of injured cells and is amenable to automation.

引用

页码：24 / 27

页数：4

共 6 条

[1]

HARVEY RWS, 1974, PUBLIC HLTH LABORATO, V8

[2]

HOPWOOD DA, 1985, GENETIC MANIPULATION, P15

[3] MODIFIED AGAR MEDIUM FOR DETECTING ENVIRONMENTAL SALMONELLAE BY THE MOST-PROBABLE-NUMBER METHOD [J].