A NEW CYCLIC-NUCLEOTIDE PHOSPHODIESTERASE ISOZYME EXPRESSED IN THE T-LYMPHOCYTE CELL-LINES

被引:43
作者
ICHIMURA, M [1 ]
KASE, H [1 ]
机构
[1] KYOWA HAKKO KOGYO CO LTD,PHARMACEUT RES LABS,1188 SHIMOTOGARI,NAGAIZUMI,SHIZUOKA 411,JAPAN
关键词
D O I
10.1006/bbrc.1993.1722
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cytosolic phosphodiesterase activities of Jurkat, a human T-lymphocyte cell line, were resolved by high performance liquid chromatography into two major peaks of cAMP-specific enzyme. One was the Rolipram-sensitive isozyme. The other showed a low Km value (0.45 ± 0.04 μM) for cAMP and different properties from reported isozymes in that it was hardly affected by well-known phosphodiesterase inhibitors or activators. This enzyme, 'JK-21', was found in other human T-cell lines, MOLT-4, HPB-ALL and HUT-78, whereas no fraction containing JK21 was detected in human B-lymphocyte cell lines, Jijoye, JY and Namalwa and in human promyelocytic cell line, HL-60. The results indicate that JK-21 is a new phosphodiesterase isozyme localized to T-cells. © 1993 Academic Press, Inc.
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页码:985 / 990
页数:6
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