COMPARISON OF EXTRACELLULAR PEROXIDASE- AND ESTERASE-DEFICIENT MUTANTS OF STREPTOMYCES-VIRIDOSPORUS T7A

被引：20

作者：

MAGNUSON, TS ^{[1
]}

CRAWFORD, DL ^{[1
]}

机构：

[1] UNIV IDAHO, INST MOLEC & AGR GENET ENGN, DEPT BACTERIOL & BIOCHEM, MOSCOW, ID 83843 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1992年 / 58卷 / 03期

关键词：

D O I：

10.1128/AEM.58.3.1070-1072.1992

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Peroxidase-deficient mutants of the lignin-degrading bacterium Streptomyces viridosporus T7A were screened for their production of acid-precipitable polymeric lignin, extracellular peroxidases and esterases, and immunoreactivities against a polyclonal antibody produced against electrophoretically purified peroxidase isoform P3 of wild-type S. viridosporus. The mutants showed diminished abilities to solubilize lignin and produce acid-precipitable polymeric lignin. Their peroxidase activities were decreased, and their esterase production patterns were altered. Western immunoblots demonstrated that the mutants produced proteins immunologically reactive with the antibody, but with different mobilities from those of wild-type proteins. These findings confirm a direct role for peroxidases in lignin solubilization. They also indicate a possible role for esterases.

引用

页码：1070 / 1072

页数：3

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