QUANTITATION OF MONOCLONAL IGM IN HYBRIDOMA CELL-CULTURE USING HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY

被引：4

作者：

SCHENERMAN, MA

COUNTRYMAN, GE

LEWIS, MA

OGRADY, EA

机构：

[1] Bio/Chem Division, Bristol-Myers Squibb Company Biotechnology Development Laboratories, Syracuse, 13221, NY

来源：

BIOTECHNOLOGY TECHNIQUES | 1993年 / 7卷 / 06期

关键词：

D O I：

10.1007/BF00155470

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Rapid, accurate, analysis of the IgM concentration in crude fermentation culture fluids is essential to provide adequate support of new culture media and cell line development. Anion-exchange chromatography had a high selectivity for IgM, allowing it to be resolved from other culture additives. When the protein was quantitated using detection at 214 nm, the procedure was linear, reproducible, accurate, sensitive (to 10 mug/ml), and was unaffected by the tissue culture additives mentioned above.

引用

页码：401 / 406

页数：6

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[No title captured]

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