CHAPERONINS DEPENDENT INCREASE OF CU,ZN SUPEROXIDE-DISMUTASE PRODUCTION IN ESCHERICHIA-COLI

被引：10

作者：

BATTISTONI, A

CARRI, MT

STEINKUHLER, C

ROTILIO, G

机构：

[1] UNIV ROMA TOR VERGATA,DEPT BIOL,VIA RIC SCI,I-00173 ROME,ITALY

[2] CNR,CTR MOLEC BIOL,ROME,ITALY

来源：

FEBS LETTERS | 1993年 / 322卷 / 01期

关键词：

CU; ZN SUPEROXIDE DISMUTASE; CHAPERONIN; PROTEIN FOLDING;

D O I：

10.1016/0014-5793(93)81099-L

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Over-expression of the chaperonins GroEL and GroES significantly suppressed the temperature-dependent pattern of expression of Cu,Zn superoxide dismutases in Escherichia coli and increased the yield of active enzyme. The results obtained indicate that chaperonins prevent degradation of metal-deficient enzyme molecules. GroEL was shown to form a complex with unfolded Cu,Zn superoxide dismutase in vitro, confirming that GroEL can interact with beta-stranded proteins.

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页码：6 / 9

页数：4

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