PHOSPHORAMIDON INHIBITS THE INTRACELLULAR CONVERSION OF BIG ENDOTHELIN-1 TO ENDOTHELIN-1 IN CULTURED ENDOTHELIAL-CELLS

被引：91

作者：

SAWAMURA, T

KASUYA, Y

MATSUSHITA, Y

SUZUKI, N

SHINMI, O

KISHI, N

SUGITA, Y

YANAGISAWA, M

GOTO, K

MASAKI, T

KIMURA, S

机构：

[1] UNIV TSUKUBA,INST BASIC MED SCI,DEPT BIOCHEM,TSUKUBA,IBARAKI 305,JAPAN

[2] TAKEDA CHEM IND LTD,TSUKUBA RES LABS,TSUKUBA,IBARAKI 30042,JAPAN

[3] UNIV TSUKUBA,INST BASIC MED SCI,DEPT PHARMACOL,TSUKUBA,IBARAKI 305,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 174卷 / 02期

关键词：

D O I：

10.1016/0006-291X(91)91485-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Effects of various protease inhibitors on the conversion of big endothelin (ET)-1 to ET-1 in cultured endothelial cells were analyzed. A metal protease inhibitor, phosphoramidon, decreases the amount of ET-1 and increases that of big ET-1 released. This effect is dose-dependent and not nonspecific. When the contents of ET-1 and big ET-1 in the cells after culturing in the medium with or without phosphoramidon were measured, the ratio of ET-1 : big ET-1 in the cells was 3.3 : 1 and phosphoramidon inverted the ratio in the cells to 1 : 3.5. These data strongly suggest that a phosphoramidon-sensitive protease converts big ET-1 to mature ET-1 intracellularly. © 1991.

引用

收藏

页码：779 / 784

页数：6

相关论文

共 14 条

[1] PHOSPHORAMIDON, A METALLOPROTEINASE INHIBITOR, SUPPRESSES THE SECRETION OF ENDOTHELIN-1 FROM CULTURED ENDOTHELIAL-CELLS BY INHIBITING A BIG ENDOTHELIN-1 CONVERTING ENZYME [J].

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[9] ANALYSIS OF ENDOTHELIN RELATED PEPTIDES IN CULTURE SUPERNATANT OF PORCINE AORTIC ENDOTHELIAL-CELLS - EVIDENCE FOR BIOSYNTHETIC-PATHWAY OF ENDOTHELIN-1 [J].

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[10] CHARACTERIZATION OF ENDOTHELIN CONVERTING ENZYME-ACTIVITIES IN SOLUBLE FRACTION OF BOVINE CULTURED ENDOTHELIAL-CELLS [J].

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