DIFFERENTIAL REGULATION OF NA-K-ATPASE ISOFORM GENE-EXPRESSION BY T3 DURING RAT-BRAIN DEVELOPMENT

被引:37
作者
CORTHESYTHEULAZ, I
MERILLAT, AM
HONEGGER, P
ROSSIER, BC
机构
[1] UNIV LAUSANNE,INST PHARMACOL,BUGNON 27,CH-1005 LAUSANNE,SWITZERLAND
[2] UNIV LAUSANNE,INST PHYSIOL,CH-1005 LAUSANNE,SWITZERLAND
来源
AMERICAN JOURNAL OF PHYSIOLOGY | 1991年 / 261卷 / 01期
关键词
MESSENGER RIBONUCLEIC ACID STABILITY; ORGANOTYPIC CELL CULTURE; SODIUM PUMP; TRIIODOTHYRONINE;
D O I
10.1152/ajpcell.1991.261.1.C124
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
A fetal rat telencephalon organotypic cell culture system was found to reproduce the developmental pattern of Na-K-adenosinetriphosphatase (ATPase) gene expression observed in vivo [Am. J. Physiol. 258 (Cell Physiol. 27): C1062-C1069, 1990]. We have used this culture system to study the effects of triiodothyronine (T3; 0.003-30 nM) on mRNA abundance and basal transcription rates of Na-K-ATPase isoforms. Steady-state mRNA levels were low at culture day 6 (corresponding to the day of birth) but distinct for each isoform alpha-3 >> beta-1 = beta-2 > alpha-2 > alpha-1. At culture day 6, T3 did not modify mRNA abundance of any isoform. At culture day 12 (corresponding to day 7 postnatal), T3 increased the mRNA level of alpha-2 (4- to 7-fold), beta-2 (4- to 5-fold), alpha-1 (3- to 6-fold), and beta-1 (1.5-fold), whereas alpha-3 mRNA levels remained unchanged. Interestingly, the basal transcription rate for each isoform differed strikingly (alpha-2 > alpha-1 >> beta-1 = beta-2 > alpha-3) but remained stable throughout 12 days of culture and was not regulated by T3. Thus we observed an inverse relationship between rate of transcription and rate of mRNA accumulation for each alpha-isoform, suggesting that alpha-1- and alpha-2-mRNA are turning over rapidly whereas alpha-3-mRNA is turning over slowly. Our data indicate that one of the mechanisms by which T3 selectively controls Na-K-ATPase gene expression during brain development in vitro occurs at the posttranscriptional level.
引用
收藏
页码:C124 / C131
页数:8
相关论文
共 34 条
[1]   EFFECT OF THYROID STATUS ON THE DEVELOPMENT OF THE DIFFERENT MOLECULAR-FORMS OF NA+,K+-ATPASE IN RAT-BRAIN [J].
ATTERWILL, CK ;
REID, J ;
ATHAYDE, CM .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1985, 40 (2-3) :149-158
[2]   EFFECT OF THYROID-HORMONE AND SERUM ON THE DEVELOPMENT OF NA+,K+-ADENOSINE TRIPHOSPHATASE AND ASSOCIATED ION FLUXES IN CULTURES FROM RAT-BRAIN [J].
ATTERWILL, CK ;
ATKINSON, DJ ;
BERMUDEZ, I ;
BALAZS, R .
NEUROSCIENCE, 1985, 14 (01) :361-&
[3]   EFFECT OF THYROID HORMONE ON BIOCHEMICAL MATURATION OF RAT BRAIN - POSTNATAL CELL FORMATION [J].
BALAZS, R ;
KOVACS, S ;
COCKS, WA ;
JOHNSON, AL ;
EAYRS, JT .
BRAIN RESEARCH, 1971, 25 (03) :555-&
[4]   POLY(A), POLY(A) BINDING-PROTEIN AND THE REGULATION OF MESSENGER-RNA STABILITY [J].
BERNSTEIN, P ;
ROSS, J .
TRENDS IN BIOCHEMICAL SCIENCES, 1989, 14 (09) :373-377
[5]   EFFECT OF THYROID-HORMONE ON THE ABUNDANCE OF NA,K-ADENOSINE TRIPHOSPHATASE ALPHA-SUBUNIT MESSENGER-RIBONUCLEIC-ACID [J].
CHAUDHURY, S ;
ISMAILBEIGI, F ;
GICK, GG ;
LEVENSON, R ;
EDELMAN, IS .
MOLECULAR ENDOCRINOLOGY, 1987, 1 (01) :83-89
[6]   NA+-K+-ATPASE GENE-EXPRESSION DURING INVITRO DEVELOPMENT OF RAT FETAL FOREBRAIN [J].
CORTHESYTHEULAZ, I ;
MERILLAT, AM ;
HONEGGER, P ;
ROSSIER, BC .
AMERICAN JOURNAL OF PHYSIOLOGY, 1990, 258 (06) :C1062-C1069
[7]  
CORTHESYTHEULAZ I, 1990, PROG CELL R, V1, P241
[8]   EFFECT OF HYDROCORTISONE AND THYROXINE ON ATPASE ACTIVITIES OF NEURONAL AND GLIAL-CELL LINES IN CULTURE [J].
ELKOUBY, A ;
LEDIG, M ;
MANDEL, P .
NEUROCHEMICAL RESEARCH, 1982, 7 (04) :387-397
[9]  
GICK GG, 1988, J BIOL CHEM, V263, P16610
[10]   THE ADHESION MOLECULE ON GLIA (AMOG) IS A HOMOLOG OF THE BETA-SUBUNIT OF THE NA,K-ATPASE [J].
GLOOR, S ;
ANTONICEK, H ;
SWEADNER, KJ ;
PAGLIUSI, S ;
FRANK, R ;
MOOS, M ;
SCHACHNER, M .
JOURNAL OF CELL BIOLOGY, 1990, 110 (01) :165-174