THERMOLUMINESCENCE EVIDENCE FOR LIGHT-INDUCED OXIDATION OF TYROSINE AND HISTIDINE-RESIDUES IN MANGANESE-DEPLETED PHOTOSYSTEM-II PARTICLES

被引:25
作者
ALLAKHVERDIEV, SI [1 ]
KLIMOV, VV [1 ]
DEMETER, S [1 ]
机构
[1] HUNGARIAN ACAD SCI,BIOL RES CTR,INST PLANT PHYSIOL,H-6701 SZEGED,HUNGARY
关键词
HISTIDINE; TYROSINE; PHOTOSYSTEM-II; ELECTRON TRANSPORT; MANGANESE-DEPLETION; THERMOLUMINESCENCE;
D O I
10.1016/0014-5793(92)80325-B
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the thermoluminescence (TL) glow curve of photosystem II, particles depleted of manganese, a tyrosine modifier, 7-chloro-4-nitrobenz-2-oxa-1,3-diazole (NBD) abolishes the TL band appearing around -55-degrees-C (TL-55). Addition of a histidine modifier, diethylpyrocarbonate results in the disappearance of the band peaking around -30-degrees-C (TL-30). NBD treatment also abolishes the EPR signal II(fast) of oxidized tyrosine donor, Y(z), and inhibits the electron transport from diphenylcarbazide to 2,6-dichlorophenol-indophenol. It is concluded that the TL-55 and TL-30 bands can be assigned to oxidized tyrosine (Y(z)+) and histidine (His+) residues, respectively, which participate in electron transfer from manganese to the reaction center of chlorophyll, P680+.
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页码:51 / 54
页数:4
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