NUCLEOTIDE-SEQUENCE OF CDNA-ENCODING ZP3-ALPHA, A SPERM-BINDING GLYCOPROTEIN FROM ZONA-PELLUCIDA OF PIG OOCYTE

被引：86

作者：

YUREWICZ, EC ^{[1
]}

HIBLER, D ^{[1
]}

FONTENOT, GK ^{[1
]}

SACCO, AG ^{[1
]}

HARRIS, J ^{[1
]}

机构：

[1] ZONAGEN,THE WOODLANDS,TX

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1174卷 / 02期

关键词：

ZONA-PELLUCIDA; CDNA; OOCYTE; SPERM-BINDING PROTEIN; CDNA SEQUENCE; NUCLEOTIDE SEQUENCE; (PIG);

D O I：

10.1016/0167-4781(93)90119-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We isolated a cDNA encoding the pig oocyte zona pellucida protein ZP3alpha from a pig ovary lambdagt11 cDNA library. The 1699 bp cDNA contains a short 3' untranslated region characteristic of cDNAs encoding zona proteins. The deduced amino acid sequence for ZP3alpha consists of 536 amino acid residues and shares 66% overall identity with a 55 kDa rabbit zona protein. Important features of the ZP3alpha polypeptide include a predicted N-terminal signal sequence, twenty-two cysteine residues, an 0-glycosylated domain and potential attachment sites for five N-linked sugar chains. A multibasic tetrapeptide occurs upstream of a predicted C-terminal transmembrane sequence; this suggests proteolytic processing of an integral membrane precursor within the constitutive secretory pathway.

引用

页码：211 / 214

页数：4

共 21 条

[1] IDENTIFICATION OF A SECONDARY SPERM RECEPTOR IN THE MOUSE EGG ZONA PELLUCIDA - ROLE IN MAINTENANCE OF BINDING OF ACROSOME-REACTED SPERM TO EGGS [J].