CLONING AND SEQUENCING OF THE RAT PREPROEPIDERMAL GROWTH-FACTOR CDNA - COMPARISON WITH MOUSE AND HUMAN SEQUENCES

被引:52
作者
SAGGI, SJ
SAFIRSTEIN, R
PRICE, PM
机构
[1] CUNY MT SINAI SCH MED, DEPT MED, DIV RENAL, BOX 1243, 1 GUSTAVE LEVY PL, NEW YORK, NY 10029 USA
[2] CUNY MT SINAI SCH MED, DEPT BIOCHEM, NEW YORK, NY 10029 USA
关键词
D O I
10.1089/dna.1992.11.481
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have cloned and sequenced the cDNA corresponding to the rat preproepidermal growth factor (ppEGF) mRNA. The cDNA contained 4,801 nucleotides, similar to that reported for the mouse (4,749 nucleotides) and the human mRNAs (4,871 nucleotides). The predicted protein sequence would contain 1,133 amino acids, smaller than that reported for the mouse (1,217 amino acids) and the human sequences (1,207 amino acids). The results of the sequencing of several cDNA clones suggested the existence of more than one structural gene for ppEGF. In addition, there was an occurrence of alternative splicing events, resulting in deletions of entire exons from the mature mRNA. These alternative splicing events do not create frameshift mutations but cause a deletion of one or more of the "EGF-like" repeat units from the ppEGF. There is approximately the same homology between the rat and mouse amino acid sequences both in the EGF region and in the other regions of the ppEGF protein. We conclude that, because of this conservation of homology, there may be an important function performed by these other regions of the ppEGF besides their function as a precursor for the EGF protein.
引用
收藏
页码:481 / 487
页数:7
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