REGIOSELECTIVE MODIFICATION OF LYSINES AMINO-GROUPS BY PROTEASES

被引：7

作者：

KITAGUCHI, H ^{[1
]}

ONO, M ^{[1
]}

ITOH, I ^{[1
]}

KLIBANOV, AM ^{[1
]}

机构：

[1] MIT,DEPT CHEM,CAMBRIDGE,MA 02139

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1991年 / 55卷 / 12期

关键词：

D O I：

10.1080/00021369.1991.10857914

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The regioselectivity of six serine proteases for the amino groups of lysine was investigated. Alpha-Chymotrypsin showed a preference for the alpha-amino group, although the selectivity can be varied 10-fold depending on the reaction medium. Subtilisin Carlsberg and other bacterial proteases were highly specific for the epsilon-amino group, regardless of the reaction medium: they were used as catalysts for the preparative synthesis of isopeptides in anhydrous tert-amyl alcohol.

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页码：3067 / 3073

页数：7

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