THE STABILITY AND FUNCTIONAL-PROPERTIES OF PROTEOLIPOSOMES MIXED WITH DEXTRAN DERIVATIVES BEARING HYDROPHOBIC ANCHOR GROUPS

被引：26

作者：

ELFERINK, MGL ^{[1
]}

DEWIT, JG ^{[1
]}

TVELD, GI ^{[1
]}

REICHERT, A ^{[1
]}

DRIESSEN, AJM ^{[1
]}

RINGSDORF, H ^{[1
]}

KONINGS, WN ^{[1
]}

机构：

[1] UNIV MAINZ,INST ORGAN CHEM,W-6500 MAINZ,GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1106卷 / 01期

关键词：

PROTEOLIPOSOME; INTEGRAL MEMBRANE PROTEIN; DEXTRAN DERIVATIVE; HYDROPHOBIC ANCHOR GROUP;

D O I：

10.1016/0005-2736(92)90217-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Liposomes composed of Escherichia coli phospholipid were coated with polysaccharides bearing hydrophobic palmitoyl anchors. The effect on the stability of liposomes without or with integral membrane proteins was investigated. A high concentration of hydrophobized dextrans protected the liposomes against detergent degradation, decreased the fluidity of the membranes, prevented fusion of the liposomes and enhanced their stability. Proteoliposomes containing beef heart cytochrome-c oxidase and the lactose transport carrier of E. coli were similarly affected by coating with the dextrans. Under these conditions both membrane proteins were still active. Long-term stability of the coated liposomes was obtained only in the absence of the integral membrane proteins.

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页码：23 / 30

页数：8

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