ENZYME CAPTURE ASSAY FOR RAPID IDENTIFICATION OF ESCHERICHIA-COLI IN BLOOD CULTURES

被引:9
作者
HUANG, SW
WU, JJ
CHANG, TC
机构
[1] FOOD IND RES & DEV INST,HSINCHU 300,TAIWAN
[2] NATL CHENG KUNG UNIV,COLL MED,DEPT MED TECHNOL,TAINAN 700,TAIWAN
关键词
D O I
10.1128/JCM.32.6.1444-1448.1994
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
An enzyme capture assay (ECA) for rapid identification of Escherichia coli in blood cultures by using beta-D-glucuronidase as a marker was developed. Microdilution plates coated with antiglucuronidase were used to capture this enzyme from the cell lysates of blood cultures which showed growth of gram-negative bacteria. The assay, using 4-methylumbelliferyl-beta-D-glucuronide as a fluorogenic substrate, had a detection limit of 0.1 ng/ml (3 x 10(-13) M) for the enzyme; this was approximately equal to a cell concentration of 10(6) CFU off. coli per ml. Among 212 blood cultures shelving growth of gram-negative bacteria, 77 specimens were found to contain E. coli by conventional culture procedures and 73 samples were positive by ECA. Among the 135 blood cultures from which E. coli was not isolated, ECA gave one false-positive (Salmonella enteritidis) reaction. Thus, the sensitivity and specificity for the identification off. coli in blood cultures by ECA were 94.8% (73/77) and 99.3% (134/135), respectively. From the finding of positive growth in the culture bottle, the assay can be completed within 4 h. In view of the high rate of isolation of E. coli from bacteremic patients, the test can be performed in parallel with conventional culture protocols; this may shorten the identification time for E. coli, and proper antimicrobial treatments may be started 24 h earlier than when results of conventional identification systems are used.
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页码:1444 / 1448
页数:5
相关论文
共 32 条
[1]   ONE-STEP PURIFICATION OF ESCHERICHIA-COLI BETA-GLUCURONIDASE [J].
BLANCO, C ;
NEMOZ, G .
BIOCHIMIE, 1987, 69 (02) :157-161
[2]   CHARACTERIZATION OF ESCHERICHIA-COLI ISOLATED IN BLOOD, URINE AND FECES FROM BACTEREMIC PATIENTS AND POSSIBLE SPREAD OF INFECTION [J].
BRAUNER, A ;
KAIJSER, B ;
WRETLIND, B ;
KUHN, I .
APMIS, 1991, 99 (04) :381-386
[3]  
CHAING TM, 1991, CHIN MED J TAIPEI, V47, P39
[4]  
Chaisson R E, 1988, Infect Dis Clin North Am, V2, P475
[5]   DEVELOPMENT OF A LATEX AGGLUTINATION-TEST FOR THE RAPID IDENTIFICATION OF VIBRIO-PARAHAEMOLYTICUS [J].
CHANG, TC ;
CHEN, CH ;
CHEN, HC .
JOURNAL OF FOOD PROTECTION, 1994, 57 (01) :31-35
[6]   RAPID DETECTION OF ESCHERICHIA-COLI IN URINE SAMPLES BY A NEW CHROMOGENIC BETA-GLUCURONIDASE ASSAY [J].
DELISLE, GJ ;
LEY, A .
JOURNAL OF CLINICAL MICROBIOLOGY, 1989, 27 (04) :778-779
[7]   SALMONELLA BACTEREMIA IN AFRICAN PATIENTS WITH HUMAN IMMUNODEFICIENCY VIRUS-INFECTION [J].
DEWIT, S ;
TAELMAN, H ;
VANDEPERRE, P ;
ROUVROY, D ;
CLUMECK, N .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1988, 7 (01) :45-47
[8]   RAPID AND ECONOMICAL IDENTIFICATION AND ANTI-MICROBIAL SUSCEPTIBILITY TEST METHODOLOGY FOR URINARY-TRACT PATHOGENS [J].
EDBERG, SC ;
TREPETA, RW .
JOURNAL OF CLINICAL MICROBIOLOGY, 1983, 18 (06) :1287-1291
[9]   FLUOROGENIC ASSAYS FOR IMMEDIATE CONFIRMATION OF ESCHERICHIA-COLI [J].
FENG, PCS ;
HARTMAN, PA .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1982, 43 (06) :1320-1329
[10]   BACTEREMIA IN CHARLESTON COUNTY, SOUTH-CAROLINA [J].
FILICE, GA ;
VANETTA, LL ;
DARBY, CP ;
FRASER, DW .
AMERICAN JOURNAL OF EPIDEMIOLOGY, 1986, 123 (01) :128-136