ENZYMATIC DETERMINATION OF TRIGLYCERIDE, FREE-CHOLESTEROL, AND TOTAL CHOLESTEROL IN TISSUE LIPID EXTRACTS

被引：494

作者：

CARR, TP ^{[1
]}

ANDRESEN, CJ ^{[1
]}

RUDEL, LL ^{[1
]}

机构：

[1] WAKE FOREST UNIV, BOWMAN GRAY SCH MED, DEPT BIOCHEM, WINSTON SALEM, NC 27157 USA

来源：

CLINICAL BIOCHEMISTRY | 1993年 / 26卷 / 01期

关键词：

TRIGLYCERIDES; CHOLESTEROL; CHOLESTERYL ESTERS; TISSUE LIPIDS; ENZYMATIC ASSAY;

D O I：

10.1016/0009-9120(93)90015-X

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Triglyceride, free cholesterol, and total cholesterol were quantified in lipid extracts of liver and thoracic aorta from nonhuman primates using commercially available enzymatic reagents. Lipids were solubilized in water by the addition of Triton X-100. Results of the enzymatic assays compared favorably with chemical assays of lipids separated by thin-layer chromatography. In addition to saving time, the present method has the advantage of measuring each lipid class from a single sample preparation. Furthermore, the procedure has been adapted for use with microtiter plates that conserve both sample and reagent.

引用

页码：39 / 42

页数：4

共 9 条

[1] ALLAIN CC, 1974, CLIN CHEM, V20, P470
[2] SENSITIVE ENZYMATIC METHOD FOR DETERMINATION OF FREE AND ESTERIFIED TISSUE CHOLESTEROL
CARLSON, SE
GOLDFARB, S
[J]. CLINICA CHIMICA ACTA, 1977, 79 (03) : 575 - 582
[3] DEHOFF JL, 1978, CLIN CHEM, V24, P433
[4] FOLCH J, 1957, J BIOL CHEM, V226, P497
[5] FOSSATI P, 1982, CLIN CHEM, V28, P2077
[6] ROESCHLA.P, 1974, Z KLIN CHEM KLIN BIO, V12, P226
[7] RUDEL LL, 1973, J LIPID RES, V14, P364
[8] A SENSITIVE ENZYMATIC ASSAY FOR DETERMINATION OF CHOLESTEROL IN LIPID EXTRACTS
SALE, FO
MARCHESINI, S
FISHMAN, PH
BERRA, B
[J]. ANALYTICAL BIOCHEMISTRY, 1984, 142 (02) : 347 - 350
[9] SARDESAI WM, 1968, CLIN CHEM, V14, P156

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