Induction of cyclooxygenase-2 mRNA and protein following transient focal ischemia in the rat brain

被引：110

作者：

Planas, AM ^{[1
]}

Soriano, MA ^{[1
]}

RodriguezFarre, E ^{[1
]}

Ferrer, I ^{[1
]}

机构：

[1] UNIV BARCELONA,HOSP PRINCEPS ESPANYA,SERV ANAT PATHOL,UNITAT NEUROPATOL,BARCELONA,SPAIN

来源：

NEUROSCIENCE LETTERS | 1995年 / 200卷 / 03期

关键词：

cyclooxygenase-2; heat-shock protein-70; middle cerebral artery occlusion; stroke; in situ hybridization; immunohistochemistry;

D O I：

10.1016/0304-3940(95)12108-G

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Expression of cyclooxygenase-2 (cox-2) mRNA and inducible heat-shock protein-70 (hsp-70) mRNA was studied with in situ hybridization techniques at 30 min and 4 h following 1 h transient middle cerebral artery (MCA) occlusion in the rat brain. In addition, immunoreactivity for cox-2 was studied after 8 h of reperfusion. Induction of hsp-70 and cox-2 mRNA was found in the brain side ipsilateral to MCA occlusion. Hsp-70 mRNA was induced in the parietal cortex and striatum within the territory of the occluded MCA. Induction of cox-2 mRNA was particularly seen in cortical layer II in the brain side ipsilateral to MCA occlusion. At 30 min of reperfusion, areas showing cox-2 mRNA induction included the cingulate and frontal cortices located perifocally to the areas showing hsp-70 mRNA induction, and the piriform cortex. At 4 h of reperfusion, induction of cox-2 mRNA was seen within the parietal cortex. At 8 h of reperfusion, immunoreactivity for cox-2 was mainly seen in the ipsilateral cortex. These results demonstrate that transient focal ischemia induces the expression of cox-2 mRNA and protein in discrete areas of the rat brain during reperfusion, which might lead to local increases of arachidonic acid metabolism

引用

页码：187 / 190

页数：4

共 29 条

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