MOUSE STRAIN DIFFERENCES IN RESIDENT PERITONEAL-CELLS - A FLOW CYTOMETRIC ANALYSIS

A flow-cytometric study of resident peritoneal cells among 8 mouse strains showed a more than twofold variation in the ratio of macrophages to macrophages plus lymphocytes, ranging from 27% in A/J to 62% in C57B/L10, with significant strain differences in a number of other cellular parameters. There was a particular deficiency of lymphocytes in strain CBA/N, which carries the xid mutation. Studies of the phagocytosis of fluorescent beads also revealed large differences in the number of beads taken up, ranging from 0·99 per cell in MFI to 1·64 per cell in BALB/c mice in a 20-min period. The total number of peritoneal cells collected also varied between strains, ranging from 2·75 × 106 in CBA/Ca to 5·85 × 106 in MFI. The total yield of macrophages per mouse ranged from 0·93 × 106 in A/J to 3·16 × 106 in C57BL/10. These differences should be taken into account when designing experiments which use resident peritoneal cells. © 1990, Royal Society of Medicine Press. All rights reserved.