AFRICAN CASSAVA MOSAIC VIRUS-DI DNA INTERFERES WITH THE REPLICATION OF BOTH GENOMIC COMPONENTS

被引:56
作者
FRISCHMUTH, T [1 ]
STANLEY, J [1 ]
机构
[1] JOHN INNES INST,JOHN INNES CTR PLANT SCI RES,DEPT VIRUS RES,COLNEY LANE,NORWICH NR4 7UH,NORFOLK,ENGLAND
关键词
D O I
10.1016/0042-6822(91)90982-H
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Natural infections of the geminivirus African cassava mosaic virus (ACMV) are known to be associated with low levels of defective interfering (DI) DNAs. Recently it has been demonstrated that extrachromosomal copies of the DI DNA, mobilized and amplified from an integrated DI DNA dimer, can ameliorate ACMV symptoms in transformed Nicotiana benthamiana, providing a possible means for the control of cassava mosaic disease. To further understand the molecular basis of the interference phenomenon, we have compared the ability of ACMV and tomato golden mosaic virus (TGMV) genomic components to replicate in leaf discs derived from DI DNA-transformed and control plants. Results indicate that the ACMV DI DNA interferes with the replication of both genomic components of ACMV to a similar extent. TGMV DNA A replicates to normal levels in transformed leaf discs and plants because it is unable to mobilize and amplify ACMV DI DNA. Differences in the relative levels of ACMV genomic components in transformed leaf discs and plants are discussed in terms of DNA replication and the availability of the genomic components for spread throughout the plant. © 1991.
引用
收藏
页码:539 / 544
页数:6
相关论文
共 24 条
[1]   BINARY AGROBACTERIUM VECTORS FOR PLANT TRANSFORMATION [J].
BEVAN, M .
NUCLEIC ACIDS RESEARCH, 1984, 12 (22) :8711-8721
[2]   ALKALINE TRANSFER OF DNA TO PLASTIC MEMBRANE [J].
CHOMCZYNSKI, P ;
QASBA, PK .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1984, 122 (01) :340-344
[3]   CASSAVA LATENT VIRUS SPECIFIC DNAS IN MOSAIC DISEASED CASSAVA OF NIGERIAN ORIGIN [J].
COUTTS, RHA ;
BUCK, KW .
NETHERLANDS JOURNAL OF PLANT PATHOLOGY, 1987, 93 (05) :241-244
[4]   GEMINIVIRUS GENES AND VECTORS [J].
DAVIES, JW ;
STANLEY, J .
TRENDS IN GENETICS, 1989, 5 (03) :77-81
[5]   BROAD HOST RANGE DNA CLONING SYSTEM FOR GRAM-NEGATIVE BACTERIA - CONSTRUCTION OF A GENE BANK OF RHIZOBIUM-MELILOTI [J].
DITTA, G ;
STANFIELD, S ;
CORBIN, D ;
HELINSKI, DR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (12) :7347-7351
[6]   DELIMITATION OF ESSENTIAL GENES OF CASSAVA LATENT VIRUS DNA-2 [J].
ETESSAMI, P ;
CALLIS, R ;
ELLWOOD, S ;
STANLEY, J .
NUCLEIC ACIDS RESEARCH, 1988, 16 (11) :4811-4829
[7]   MUTATIONAL ANALYSIS OF COMPLEMENTARY-SENSE GENES OF AFRICAN CASSAVA MOSAIC-VIRUS DNA-A [J].
ETESSAMI, P ;
SAUNDERS, K ;
WATTS, J ;
STANLEY, J .
JOURNAL OF GENERAL VIROLOGY, 1991, 72 :1005-1012
[8]   SIZE REVERSION OF AFRICAN CASSAVA MOSAIC-VIRUS COAT PROTEIN GENE DELETION MUTANTS DURING INFECTION OF NICOTIANA-BENTHAMIANA [J].
ETESSAMI, P ;
WATTS, J ;
STANLEY, J .
JOURNAL OF GENERAL VIROLOGY, 1989, 70 :277-289
[9]   COMPLETE NUCLEOTIDE-SEQUENCE OF THE INFECTIOUS CLONED DNA COMPONENTS OF TOMATO GOLDEN MOSAIC-VIRUS - POTENTIAL CODING REGIONS AND REGULATORY SEQUENCES [J].
HAMILTON, WDO ;
STEIN, VE ;
COUTTS, RHA ;
BUCK, KW .
EMBO JOURNAL, 1984, 3 (09) :2197-2205
[10]   A BINARY PLANT VECTOR STRATEGY BASED ON SEPARATION OF VIR-REGION AND T-REGION OF THE AGROBACTERIUM-TUMEFACIENS TI-PLASMID [J].
HOEKEMA, A ;
HIRSCH, PR ;
HOOYKAAS, PJJ ;
SCHILPEROORT, RA .
NATURE, 1983, 303 (5913) :179-180