AN IMPROVED METHOD FOR ISOLATING RNA FROM PORCINE ADIPOSE-TISSUE

被引：42

作者：

LOUVEAU, I ^{[1
]}

CHAUDHURI, S ^{[1
]}

ETHERTON, TD ^{[1
]}

机构：

[1] PENN STATE UNIV,DEPT DAIRY & ANIM SCI,UNIVERSITY PK,PA 16802

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 196卷 / 02期

关键词：

D O I：

10.1016/0003-2697(91)90471-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study, we describe a method that we developed to isolate total RNA from porcine adipose tissue. This method entails homogenizing porcine adipose tissue in 10 ml of 4 m guanidium thiocyanate, 25 mm sodium citrate, 0.5% Sarcosyl, 0.1 m β-mercaptoethanol, pH 7.0, and then performing two CHCl3 extractions to remove lipid before following the procedure described by P. Chomczynski and N. Sacchi (1987, Anal. Biochem. 162, 156-159). This modification improved the yield of RNA approximately threefold (yield was 88 ± 7 μg total RNA/g of tissue) without affecting RNA quality. © 1991.

引用

页码：308 / 310

页数：3

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