MATRIX METALLOPROTEINASE-2 AND TISSUE INHIBITOR OF METALLOPROTEINASE-2 EXPRESSION IN PEDIATRIC TUMOR-CELLS - EFFECTS OF TUMOR-CELL PROLIFERATION MODULATORS ON GELATINOLYTIC ACTIVITY

被引：18

作者：

DEVEAS, RG

SCHWEIGERER, L

MEDINA, MA

机构：

[1] UNIV MALAGA,FAC CIENCIAS,BIOQUIM & BIOL MOLEC LAB,E-29071 MALAGA,SPAIN

[2] CHILDRENS UNIV HOSP,DIV HEMATOL ONCOL,D-69120 HEIDELBERG,GERMANY

来源：

JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY | 1995年 / 121卷 / 05期

关键词：

72-KDA GELATINASE; TYPE IV COLLAGENASE; MMP-2; TIMP-2; NEUROBLASTOMA; OSTEOSARCOMA; RHABDOMYOSARCOMA; GENISTEIN;

D O I：

10.1007/BF01209593

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We have examined the expression of 72-kDa gelatinase/type IV collagenase or matrix metalloproteinase-2 (MMP-2) and its inhibitor, tissue inhibitor of metalloproteinase-2 (TIMP-2), in various cell lines derived from paediatric tumours. In a neuroblastoma model system of tumour progression, the expression level of MMP-2 mRNA was higher in the more malignant cell line. Surprisingly, MMP-2 was not expressed in the highly malignant rhabdomyosarcoma A-204 cell line. TIMP-2 showed higher expression levels in the 007 and U-2OS tumour cell lines than in the more malignant ones, WAC2 and A-204 cells. We have also determined the effect of some tumour cell proliferation modulators on gelatinolytic activity. While basic fibroblast growth factor and retinoic acid produced no apparent change in gelatinolytic activity, genistein induced in partial inhibition of gelatinolytic activity.

引用

页码：275 / 278

页数：4

共 35 条

[1] Akiyama T., Ishida J., Nakagawa S., Ogawara H., Watanabe S., Itoh N., Shibuya M., Fuami Y., Genistein, a specific inhibitor of tyrosine-specific protein kinases, J Biol Chem, 262, pp. 5592-5595, (1987)
[2] Basset P., Bellocq J.P., Wolf C., Stoll I., Hutin P., Limacher J.M., Podhajcer O.L., Chenard M.P., Rio M.C., Chambon P., A novel metalloproteinase gene specifically expressed in stromal cells of breast carcinomas, Nature, 348, pp. 699-704, (1990)
[3] Briozzo P., Morisset M., Capony F., Rougeot G., Rochefort H., In vitro degradation of extracellular matrix with M<sub>r</sub> 52,000 cathepsin D secreted by breast cancer cells, Cancer Res, 48, pp. 3688-3692, (1988)
[4] Burgess W.H., Maciag T., The heparin-binding (fibroblast) growth factor family of proteins, Annu Rev Biochem, 58, pp. 575-606, (1989)
[5] Chomczynski P., Sacchi N., Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction, Anal Biochem, 162, pp. 156-159, (1987)
[6] Collier I.E., Wilhelm S.M., Eisen A.Z., Marmer B.L., Grant G.A., Seltzer J.L., Kronberger A., He C., Bauer E.A., Goldberg C., H- ras oncogene transformed human bronchial epithelial cells (TBE-1) secrete a single metalloprotease capable of degrading basement membrane collagen, J Biol Chem, 263, pp. 6579-6587, (1988)
[7] Dano K., Andreasen P.A., Grondahl-Hansen J., Kristensen P., Nielsen L.S., Skriver L., Plasminogen activators, tissue degradation and cancer, Adv Cancer Res, 45, pp. 139-266, (1985)
[8] Favennec L., Cals M.J., The biological effect of retinoids on cell differentiation and proliferation, J Clin Chem Clin Biochem, 26, pp. 479-489, (1988)
[9] Folkman J., Shing Y., Angiogenesis, J Biol Chem, 267, pp. 10931-10934, (1992)
[10] Fotsis T., Pepper M., Adlercreutz H., Fleischmann G., Hase T., Montesano R., Schweigerer L., Genistein, a novel dietary-derived angiogensis inhibitor, Proc Natl Acad Sci USA, 90, pp. 2690-2694, (1993)

← 1 2 3 4 →