LEGIONELLA-PNEUMOPHILA INHIBITS SUPEROXIDE GENERATION IN HUMAN MONOCYTES VIA THE DOWN-MODULATION OF ALPHA-PROTEIN AND BETA-PROTEIN KINASE-C ISOTYPES

被引:25
作者
JACOB, T
ESCALLIER, JC
SANGUEDOLCE, MV
CHICHEPORTICHE, C
BONGRAND, P
CAPO, C
MEGE, JL
机构
[1] HOP ST MARGUERITE,IMMUNOL LAB,F-13009 MARSEILLE,FRANCE
[2] CTR REG TRANSFUS SANGUINE,F-13005 MARSEILLE,FRANCE
关键词
LEGIONELLA PNEUMOPHILA; MONOCYTES; PROTEIN KINASE C; OXIDATIVE METABOLISM;
D O I
10.1002/jlb.55.3.310
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Legionella pneumophila may subvert monocyte defenses by several mechanisms including the inhibition of phagosome-lysosome fusion or the impairment of oxidative metabolism. We have investigated the effect if L. pneumophila Knoxville 1, a virulent strain that does not inhibit phagosome-lysosome fusion, on the oxidative responsiveness of human monocytes. Infection of monocytes with L. pneumophila for 48 h resulted in marked inhibition of superoxide generation stimulated by phorbol myristate acetate (PMA) but not by zymosan, a particulate agonist. Evidence is provided that L. pneumophila interfered with the transductional pathway (i.e., protein kinase C, PRC) leading to activation of the NADPH oxidase in monocytes. The phosphorylation of 34-, 48-, 62-, 68-, and 80-kDa proteins stimulated by PMA was markedly inhibited in infected monocytes. In addition, the expression of both alpha and beta PKC isotypes was partially inhibited in infected monocytes. Taken together, our data suggest that the down-modulation of PKC isotypes plays a role in the inhibition of PMA-stimulated superoxide generation.
引用
收藏
页码:310 / 312
页数:3
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