HOST TUMOR INFILTRATING LYMPHOCYTES IN B-CELL NON-HODGKINS-LYMPHOMAS

被引：7

作者：

DIAZ, JI

EDINGER, MG

STOLER, M

TUBBS, RR

机构：

[1] Departments of Pathology, Cleveland, OH, Cleveland Clinic Foundation

[2] University of Tampa Health Sciences Center, FL

[3] H. Lee Moffitt Cancer Center, University of South Florida, FL

来源：

LEUKEMIA & LYMPHOMA | 1993年 / 9卷 / 1-2期

关键词：

NON-HODGKINS LYMPHOMAS; FLOW CYTOMETRY; IMMUNOREGULATION;

D O I：

10.3109/10428199309148508

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Tumor-infiltrating T lymphocytes (TIL-T) were quantitated by three color flow cytometry in cell suspensions from excisional biopsy specimens of 43 B cell non-Hodgkin's lymphomas (NHL) and 8 benign lymphoid hyperplasias (BLH) to identify potential differences in host T cell responses. We quantitated three TIL-T subsets: CD3+CD4+CD8- (helper-inducer), CD3+CD4-CD8+ (suppressor-cytotoxic) and CD3+CD25-HLADr+ (long term activated TIL-T) and compared them in three diagnostic groups: BLH, low grade B cell NHL (LG NHL) and intermediate-high grade B cell NHL (IG-HG NHL). The following results were obtained: Mean percentage +/- s.c. of activated TIL-T for BLH, LG-NHL and IG-HG NHL: 10.3 +/- 1.9, 23.2 +/- 4.6 and 38.8 +/- 9.5, respectively. Mean percentage +/- s.c. of suppressor-cytotoxic TIL-T for same groups: 13.9 +/- 1.5, 14.9 +/- 1.9 and 34.4 +/- 4.5, respectively. Mean percentage +/- s.e. of helper-inducer TIL-T cells for the same groups was 38.2 +/- 12.7, 32.1 +/- 7.2 and 22.5 +/- 4.6, respectively. Helper/suppressor ratio +/- s.e. for same groups was 3.0 +/- 1.1, 2.4 +/- 0.6 and 1.3 +/- 0.4, respectively. Activated and suppressor-cytotoxic TIL-T percentage progressively increased from BLH toward IG-HG NHL. The percentage of these two TIL-T subsets were significantly higher in IG-HG NHL than in BLH and LG-NHL (P < 0.0007, 0.0002, 0.0001 and 0.0260 for the comparisons TIL-T in BLH vs IG-HG NHL and LG-NHL vs IG-HG respectively). These results suggest the possibility that activated-cytotoxic TIL-T represent a host cellular immune response versus neoplastic NHL B cells and encourage future in vitro functional studies.

引用

页码：85 / 90

页数：6

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