PREPARATION AND CHARACTERIZATION OF A MOUSE MONOCLONAL-ANTIBODY AGAINST A RAT-KIDNEY PAPILLARY ANTIGEN

被引：5

作者：

HILDEBRAND, H ^{[1
]}

FALKENBERG, FW ^{[1
]}

SCHWENGBERG, S ^{[1
]}

SCHLUTER, G ^{[1
]}

BOMHARD, E ^{[1
]}

机构：

[1] BAYER AG, FACHBEREICH TOXIKOL, W-5600 WUPPERTAL 1, GERMANY

来源：

TOXICOLOGY IN VITRO | 1993年 / 7卷 / 04期

关键词：

D O I：

10.1016/0887-2333(93)90040-C

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Monoclonal antibodies were prepared in an attempt to develop diagnostic tools for the identification of toxic damage to the renal papilla. One of these antibodies, termed PAP X 5C10, recognizes an antigen that is located on the luminal side of collecting duct epithelial cells. As shown by Western blotting experiments, this antigen can be extracted from papillary tissue and visualized as a broad band of high molecular weight. Enzyme-linked immunosorbent assays have shown that increased amounts of this antigen can be detected in urine of rats treated with bromoethaneamine. This procedure thus enables this antigen to be detected also in supernatants of cultured ductal cells.

引用

页码：421 / 425

页数：5

共 5 条

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