IDENTIFICATION OF LRP-REGULATED GENES BY INVERSE PCR AND SEQUENCING - REGULATION OF 2 MAL OPERONS OF ESCHERICHIA-COLI BY LEUCINE-RESPONSIVE REGULATORY PROTEIN

被引：23

作者：

TCHETINA, E ^{[1
]}

NEWMAN, EB ^{[1
]}

机构：

[1] CONCORDIA UNIV, DEPT BIOL, MONTREAL, PQ H3G 1M8, CANADA

来源：

JOURNAL OF BACTERIOLOGY | 1995年 / 177卷 / 10期

关键词：

D O I：

10.1128/jb.177.10.2679-2683.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have used the technique of inverse PCR to identify Escherichia coli chromosomal genes carrying Lrp-regulated inserts, This technique revealed that malT, malEFG, and malB-lamB-malK are all activated two-to fivefold by Lrp and confirmed that Lrp regulates expression of the leuDBCA and livHJKG operons. lacZ transcription is also increased in the presence of Lrp, However, the growth rate of the Lrp mutant on maltose and lactose is not decreased by Lrp deficiency.

引用

页码：2679 / 2683

页数：5

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