RNA VIRUS GENOMES HYBRIDIZE TO CELLULAR RIBOSOMAL-RNAS AND TO EACH OTHER

被引:25
作者
MCCLURE, MA
PERRAULT, J
机构
[1] WASHINGTON UNIV, SCH MED, DEPT MICROBIOL & IMMUNOL, ST LOUIS, MO 63110 USA
[2] SAN DIEGO STATE UNIV, INST MOLEC BIOL, SAN DIEGO, CA 92182 USA
[3] SAN DIEGO STATE UNIV, DEPT BIOL, SAN DIEGO, CA 92182 USA
关键词
D O I
10.1128/JVI.57.3.917-921.1986
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
In this communication we show that the RNA genomes of vesicular stomatitis, Sindbis, and reoviruses can specifically hybridize under stringent conditions of the large rRNAs present in HeLa cell cytoplasmic extracts. In addition, we show that some virus genome RNAs can also hybridize to each other. On the basis of our previous detailed studied identifying specific regions of hybridization between the poliovirus genome and 28S rRNA, we suggest that a similar phenomenon of "patchy complementary" may be responsible for the interactions described here (M. A. McClure and J. Perrault, Nucleic Acids Res. 13:6797-6816, 1985). The possible biological implications of these cross-reacting hybridizations and practical considerations in the use of viral probes for diagnosis are discussed.
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页码:917 / 921
页数:5
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