2-DIMENSIONAL MICROELECTROPHORESIS IN SUPPORTED LIPID BILAYERS

被引：101

作者：

STELZLE, M ^{[1
]}

MIEHLICH, R ^{[1
]}

SACKMANN, E ^{[1
]}

机构：

[1] TECH UNIV MUNICH,W-8046 GARCHING,GERMANY

来源：

BIOPHYSICAL JOURNAL | 1992年 / 63卷 / 05期

关键词：

D O I：

10.1016/S0006-3495(92)81712-5

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We report the application of supported bilayers for two dimensional microelectrophoresis. This method allows the lateral separation and accumulation of charged amphiphilic molecular probes in bilayers by application of an electric field parallel to the bilayer surface. Diffusion coefficient and mobility of the fluorescent probes are determined by observation of the fluorescence recovery after photobleaching (pattern bleaching). The diffusion coefficients and the mobilities of oppositely charged fluorescent probes in one bilayer can be determined independently from a single measurement. By analysis of the motion of charged and uncharged probes in one membrane one can distinguish between the motion caused by the electric field acting on the charge of individual probes and that caused by frictional forces due to electroosmosis.

引用

页码：1346 / 1354

页数：9

共 13 条

[1] DIFFUSION IN QUASI 2-DIMENSIONAL MACROMOLECULAR SOLUTIONS
EGGL, P
PINK, D
QUINN, B
RINGSDORF, H
SACKMANN, E
[J]. MACROMOLECULES, 1990, 23 (14) : 3472 - 3480
[2] FALKENHAGEN H, 1971, THEORIE ELEKTROLYTE, P148
[3] Hiemenz P. C, 1986, PRINCIPLES COLLOID S
[4] Hunter R.J., 1981, ZETA POTENTIAL COLLO, DOI [10.1016/c2013-0-07389-6, 10.1016/j.berh.2014.02.004]
[5] Activities of urease and pepsin monolayers
Langmuir, I
Schaefer, VJ
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1938, 60 : 1351 - 1360
[6] THE ROLE OF ELECTROOSMOSIS IN THE ELECTRIC-FIELD-INDUCED MOVEMENT OF CHARGED MACROMOLECULES ON THE SURFACES OF CELLS
MCLAUGHLIN, S
POO, MM
[J]. BIOPHYSICAL JOURNAL, 1981, 34 (01) : 85 - 93
[7] MOLECULAR FRICTION AND EPITACTIC COUPLING BETWEEN MONOLAYERS IN SUPPORTED BILAYERS
MERKEL, R
SACKMANN, E
EVANS, E
[J]. JOURNAL DE PHYSIQUE, 1989, 50 (12): : 1535 - 1555
[8] PRESS WH, 1981, NUMERICAL RECIPES C, P542
[9] DETERMINATION OF MOLECULAR-MOTION IN MEMBRANES USING PERIODIC PATTERN PHOTOBLEACHING
SMITH, BA
MCCONNELL, HM
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (06) : 2759 - 2763
[10] SENSITIVE DETECTION OF PROTEIN ADSORPTION TO SUPPORTED LIPID BILAYERS BY FREQUENCY-DEPENDENT CAPACITANCE MEASUREMENTS AND MICROELECTROPHORESIS
STELZLE, M
SACKMANN, E
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 981 (01) : 135 - 142

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