LARGE-SCALE SYNTHESIS OF OLIGONUCLEOTIDES VIA PHOSPHORAMIDITE NUCLEOSIDES AND A HIGH-LOADED POLYSTYRENE SUPPORT

被引：52

作者：

WRIGHT, P

LLOYD, D

RAPP, W

ANDRUS, A

机构：

[1] APPL BIOSYST INC,850 LINCOLN CTR DR,FOSTER CITY,CA 94404

[2] RAPP POLYMERE,W-7400 TUBINGEN,GERMANY

来源：

TETRAHEDRON LETTERS | 1993年 / 34卷 / 21期

关键词：

D O I：

10.1016/S0040-4039(00)79159-3

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Large scale quantities of phosphodiester and phosphorothioate oligonucleotides are synthesized on an aminopolyethyleneglycol derivatized polystyrene (TentaGel) support. Efficient, automated synthesis up to 1 mmole scale is achieved with phosphoramidite nucleoside monomers and 5-ethylthiotetrazole activator.

引用

页码：3373 / 3376

页数：4

共 30 条

[1] HINDERED DIALKYLAMINO NUCLEOSIDE PHOSPHITE REAGENTS IN THE SYNTHESIS OF 2 DNA 51-MERS [J].