A SCANNING-TUNNELING-MICROSCOPY COMPARISON OF PASSIVE ANTIBODY ADSORPTION AND BIOTINYLATED ANTIBODY LINKAGE TO STREPTAVIDIN ON MICROTITER WELLS

被引：48

作者：

DAVIES, J ^{[1
]}

DAWKES, AC ^{[1
]}

HAYMES, AG ^{[1
]}

ROBERTS, CJ ^{[1
]}

SUNDERLAND, RF ^{[1
]}

WILKINS, MJ ^{[1
]}

DAVIES, MC ^{[1
]}

TENDLER, SJB ^{[1
]}

JACKSON, DE ^{[1
]}

EDWARDS, JC ^{[1
]}

机构：

[1] UNIV NOTTINGHAM,DEPT PHARMACEUT SCI,BIOL SPM LAB,NOTTINGHAM NG7 2RD,ENGLAND

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1994年 / 167卷 / 1-2期

关键词：

SCANNING TUNNELING MICROSCOPY; PASSIVE ANTIBODY ADSORPTION; BIOTINYLATED ANTIBODY LINKAGE;

D O I：

10.1016/0022-1759(94)90095-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An antiferritin antibody was either, (a) passively adsorbed to microwells or (b) biotinylated and immobilised to streptavidin coated microwells. Scanning tunnelling microscope (STM) imaging of these well surfaces coated with a platinum (95%) carbon (5%) coating (Pt/C) conductive layer showed a randomly oriented array of antibodies for passive adsorption whereas for biotin-streptavidin immobilisation there was a more uniform and even distribution of antibodies on the well surface. On further incubation with ferritin STM imaging showed that for passive adsorption approximately 5% of the surface was functional, while for the biotinylated antibody it was greater than 60%. The images presented in this paper show graphically the loss of functionality that occurs using passive adsorption and, conversely, the preservation of antibody functionality using the biotin-streptavidin linkage for antibody immobilisation. These results correlate well with the work of others in the field.

引用

页码：263 / 269

页数：7

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