RAPID METHOD FOR PREPARATION OF PURE VERATRYL ALCOHOL FOR THE ASSAY OF LIGNIN PEROXIDASE

被引：3

作者：

KIRTIKARA, K

SCHAICH, KM

FAGAN, JM

FRENKEL, C

机构：

[1] RUTGERS STATE UNIV, COOK COLL, DEPT PLANT SCI, NEW BRUNSWICK, NJ 08903 USA

[2] RUTGERS STATE UNIV, DEPT FOOD SCI, NEW BRUNSWICK, NJ 08903 USA

[3] RUTGERS STATE UNIV, DEPT ANIM SCI, NEW BRUNSWICK, NJ 08903 USA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1995年 / 23卷 / 03期

关键词：

3,4-DIMETHOXYBENZYL ALCOHOL; LIGNIN PEROXIDASE ASSAY; PHANEROCHAETE CHRYSOSPORIUM; VERATRYL ALCOHOL;

D O I：

10.1016/0167-7012(95)00037-L

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a simple method for the rapid preparation of veratryl alcohol by reducing commercially available veratraldehyde with sodium borohydride resulting in high-purity veratryl alcohol. The lag period in the activity of lignin peroxidase from Phanerochaete chrysosporium that is associated with use of commercial preparations of the substrate is eliminated with the use of pure veratryl alcohol. The compound affords also estimation of low enzymatic activity.

引用

页码：253 / 259

页数：7

共 5 条

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[2] PRODUCTION OF MULTIPLE LIGNINASES BY PHANEROCHAETE-CHRYSOSPORIUM - EFFECT OF SELECTED GROWTH-CONDITIONS AND USE OF A MUTANT STRAIN
KIRK, TK
CROAN, S
TIEN, M
MURTAGH, KE
FARRELL, RL
[J]. ENZYME AND MICROBIAL TECHNOLOGY, 1986, 8 (01) : 27 - 32
[3] OVERPRODUCTION OF LIGNIN-DEGRADING ENZYMES BY AN ISOLATE OF PHANEROCHAETE-CHRYSOSPORIUM
ORTH, AB
DENNY, M
TIEN, M
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (09) : 2591 - 2596
[4] TIEN M, 1986, J BIOL CHEM, V261, P1687
[5] TIEN M, 1988, METHOD ENZYMOL, V161, P238

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