SYNTHESIS OF DTDP-6-DEOXY-4-KETOGLUCOSE AND ANALOGS WITH NATIVE AND RECOMBINANT DTDP-GLUCOSE-4,6-DEHYDRATASE

被引：25

作者：

STEIN, A

KULA, MR

ELLING, L

VERSECK, S

KLAFFKE, W

机构：

[1] FORSCHUNGSZENTRUM JULICH, FORSCHUNGSZENTRUM, UNIV DUSSELDORF, INST ENZYMTECHNOL, D-52404 JULICH, GERMANY

[2] UNIV GESAMTHSCH WUPPERTAL, INST CHEM MIKROBIOL, W-5600 WUPPERTAL, GERMANY

[3] UNIV HAMBURG, D-20146 HAMBURG, GERMANY

[4] UNILEVER RES LABS VLAARDINGEN, BIOORGAN CHEM, 3133 AT VLAARDINGEN, NETHERLANDS

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 1995年 / 34卷 / 16期

关键词：

BIOTECHNOLOGY; CHROMATOGRAPHY; DEHYDRATASES; DEOXYCARBOHYDRATES; ENZYMES;

D O I：

10.1002/anie.199517481

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The enzymatic deoxygenation of dTDP‐glucose and two 3‐deoxy analogues [Eq. (a)] is now possible on a preparative scale with dTDP‐D‐glucose‐4,6‐dehydratase. In addition a highly selective HPLC method for the separation of structurally related activated sugars has been developed. This should provide new impetus for structure–function studies of deoxysugars and further elucidation of their biosynthetic pathways. R  OH, H, N3; dTDP = thymidine‐5′‐diphosphate. (Figure Presented.) Copyright © 1995 by VCH Verlagsgesellschaft mbH, Germany

引用

页码：1748 / 1749

页数：2

共 14 条

[1] DIRECT DETERMINATION OF UDP-GLUCURONIC ACID IN CELL-EXTRACTS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].