DETERMINATION OF THE BRANCH LOCATION OF EXTRA N-ACETYLLACTOSAMINE UNITS IN SIALO N-LINKED TETRAANTENNARY OLIGOSACCHARIDES

被引：23

作者：

HOKKE, CH

KAMERLING, JP

VANDEDEM, GWK

VLIEGENTHART, JFG

机构：

[1] STATE UNIV UTRECHT,DEPT BIOORGAN CHEM,BIJVOET CTR,POB 80075,3508 TB UTRECHT,NETHERLANDS

[2] DIOSYNTH INT BV,OSS,NETHERLANDS

来源：

FEBS LETTERS | 1991年 / 286卷 / 1-2期

关键词：

RECOMBINANT ERYTHROPOIETIN; EXTRA N-ACETYLLACTOSAMINE UNIT; H-1-NMR SPECTROSCOPY;

D O I：

10.1016/0014-5793(91)80931-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An approach is presented for the determination of the branch location of 1 or 2 extra N-acetyllactosamine units in sialo N-linked carbohydrate chains from glycoproteins. Tetraantennary oligosaccharides containing extra N-acetyllactosamine units were digested with endo-beta-galactosidase, followed by treatment with N-acetyl-beta-glucosaminidase, yielding products which could be analysed by H-1-NMR spectroscopy, thereby giving conclusive data about the location of the extra units in the intact structures.

引用

页码：18 / 24

页数：7

共 23 条

[1] THE BETA-SUBUNIT OF HUMAN CHORIONIC-GONADOTROPIN CONTAINS N-GLYCOSIDIC TRISIALO TRI-ANTENNARY AND TRI'-ANTENNARY CARBOHYDRATE CHAINS [J].