RAPID AND QUANTITATIVE-ANALYSIS OF RECOMBINANT PROTEIN EXPRESSION USING PYROLYSIS MASS-SPECTROMETRY AND ARTIFICIAL NEURAL NETWORKS - APPLICATION TO MAMMALIAN CYTOCHROME B(5) IN ESCHERICHIA-COLI

被引：25

作者：

GOODACRE, R

KARIM, A

KADERBHAI, MA

KELL, DB

机构：

[1] Institute of Biological Sciences, University of Wales, Aberystwyth

来源：

JOURNAL OF BIOTECHNOLOGY | 1994年 / 34卷 / 02期

关键词：

PYROLYSIS MASS SPECTROMETRY; ARTIFICIAL NEURAL NETWORK; RECOMBINANT PROTEIN; MAMMALIAN CYTOCHROME B(5); CHEMOMETRICS; BIOTECHNOLOGY;

D O I：

10.1016/0168-1656(94)90088-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Recombinant Escherichia coli clones encoding between 0 and 6 copies of the mammalian cytochrome b(5) gene were subjected to pyrolysis mass spectrometry (PyMS). To deconvolute the pyrolysis mass spectra so as to obtain quantitative information on the amount of cytochrome b(5) produced fully-interconnected feedforward artificial neural networks (ANNs) were studied. It was found that the combination of PyMS and ANNs could be used to predict the amount of cytochrome b(5) expressed in E. coli. PyMS is a novel, convenient and rapid method for the screening and analysis of microbial and other cultures producing recombinant proteins.

引用

页码：185 / 193

页数：9

共 42 条

[1]

Aebersol Ruedi, 1993, Current Opinion in Biotechnology, V4, P412, DOI 10.1016/0958-1669(93)90006-I

[2] EVALUATION OF A LOW-COST, AUTOMATED PYROLYSIS MASS-SPECTROMETER [J].