TROPHECTODERM BIOPSY IN HUMAN BLASTOCYSTS

被引：98

作者：

DOKRAS, A

SARGENT, IL

ROSS, C

GARDNER, RL

BARLOW, DH

机构：

[1] IVF Unit, Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital

[2] ICRF Developmental Biology Unit, Department of Zoology

来源：

HUMAN REPRODUCTION | 1990年 / 5卷 / 07期

关键词：

Human blastocysts; Preimplantation diagnosis; Trophectoderm biopsy; Zona dissection;

D O I：

10.1093/oxfordjournals.humrep.a137191

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Trophectoderm biopsy was carried out on 47 human blastocysts. A slit was made in the zona pellucida opposite the inner cell mass by micromanipulative techniques. The human blastocyst zona offered more resistance to slitting compared to that of the mouse. After 18-24 h, controlled herniation of the trophectoderm cells was observed. These cells were biopsied when the diameter of the herniation was aproximately equal to that of the blastocyst. The size of the slit and the stage of embryonic development at which slitting was performed were important for successful herniation to occur. After slitting, 76% of day 5-6 blastocysts showed herniation whilst only 42% of day 7-8 blastocysts herniated. Further development of the manipulated embryos was not apparently impaired, as hatching occurred in 44% of the former and 20% of the latter, compared with 18.1% in nonmanipulated controls. The biopsied cells (∼ 10-30) usually remained in a chunp but 14% formed vesicles on the day after biopsy. There was, however, no evidence of adherence to the dish or formation of monolayers. These results demonstrate the feasibility of trophectaderm biopsy in human blastocysts and that sufficient extra-embryonic material can be obtained by this technique for preimplantation diagnosis of genetic disorders. © 1990 Oxford University Press.

引用

页码：821 / 825

页数：5

共 16 条

[1] Betteridge K.J., Hare W.C.D., Singh E.L., Approaches to Sex Selection in Farm Animals, pp. 109-125, (1981)
[2] Cohen J., Eisner C., Kort H., Malter H., Massey J., Mayer M.P., Wiemer K., Impairment of the hatching process following IVF in the human and improvement of implantation by assisting hatching using micromanipulation, Hum. Reprod., 5, pp. 7-13, (1990)
[3] Gardner R.L., Manipulations on the blastocyst, Adv., 6, pp. 279-296, (1971)
[4] Gardner R.L., Edwards R.G., Control of the sex ratio at full term in the rabbit by transferring sexed blastocysts, Nature, pp. 218-349, (1968)
[5] Gardner R.L., Papioannou V.E., Barton S.C., Origin of the ectoplacental cone and secondary giant cells in mouse blastocysts reconstituted from isolated trophoblast and inner cell mass, J. Em Bryol. Exp. Morphol., 30, pp. 561-572, (1973)
[6] Handyside A.H., Penketh R.J.A., Winston R.M.L., Pattison J.K., Delhanty J.D.A., Tuddenham E.G.D., Biopsy of pre implantation embryos and sexing by DNA amplification, Lancet, 1, (1989)
[7] Monk M., Handyside A., Hardy K., Whittingham D.G., Preimplantation diagnosis of deficiency of hypoxanthine phos phoribosyl transferase in a mouse model for Lesch Nyhan syndrome, Lancet, 2, pp. 423-425, (1987)
[8] Monk M., Muggleton-Harris A., Rawlings E., Whittingham D.G., Preimplantation diagnosis of HPRT-deficient male, and carrier female mouse embryos by trophectoderm biopsy, Hum. Reprod., 3, pp. 377-381, (1988)
[9] Nichols J., Gardner R.L., Heterogenous differentiation of external cells in individual isolated early mouse inner cell masses in culture, J. Embryol. Exp. Morphol., 80, pp. 225-240, (1984)
[10] Nijs M., Van Steirteghem A.C., Assessment of different isolation procedures for blastomeres from two cell mouse embryos, Hum. Reprod., 2, pp. 421-424, (1987)

← 1 2 →