MOLECULAR-CLONING OF DNA FROM A SORTED HUMAN MINICHROMOSOME

被引：5

作者：

FERRETTI, L

RAIMONDI, E

GAMBERI, C

YOUNG, BD

DECARLI, L

SGARAMELLA, V

机构：

[1] CNR,IST DIFESA & VALORIZZAZ GERMOPLASMA,I-20133 MILAN,ITALY

[2] ST BARTHOLOMEWS HOSP,IMPERIAL CANC RES FUND,DEPT MED ONCOL,LONDON EC1A 7BE,ENGLAND

[3] UNIV CALABRIA,DIPARTIMENTO BIOL CELLULARE,I-87036 RENDE,ITALY

来源：

GENE | 1991年 / 99卷 / 02期

关键词：

RECOMBINANT DNA; GENOMIC CLONING; PHAGE-LAMBDA VECTOR; LOW-COPY-NUMBER DNA CLONES; INSITU HYBRIDIZATION; CHROMOSOME-9;

D O I：

10.1016/0378-1119(91)90131-T

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A human supernumerary minichromosome (MC), found in a newborn baby and sorted on a fluorescence-activated cell sorter (FACS-440) has been previously described [Ferretti et al., Cytotechnology 1 (1987) 7-12]. We report here on the construction of a library of EcoRI fragments in the phage lambda-gtWES. lambda-B', starting from 7.5 ng of MC DNA, and describe the isolation of single-copy DNA clones from the library in a two-step procedure. We employed in situ hybridization to unambiguously select the clones specific for the MC, and used three of these clones to demonstrate that it originated from chromosome 9.

引用

页码：229 / 234

页数：6

共 21 条

[1]

BARDONI B, 1988, Genomics, V3, P32, DOI 10.1016/0888-7543(88)90155-3

[2] SCREENING GAMMAGT RECOMBINANT CLONES BY HYBRIDIZATION TO SINGLE PLAQUES INSITU [J].