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PRODUCTION OF ALPHA-ISOPROPYLMALATE AND BETA-ISOPROPYLMALATE BY A MUTANT FROM CANDIDA-MALTOSA
被引：4
作者：
BODE, R
SAMSONOVA, IA
BIRNBAUM, D
机构：
来源：
ZENTRALBLATT FUR MIKROBIOLOGIE
|
1991年
/
146卷
/
01期
关键词：
CANDIDA-MALTOSA;
LEUCINE BIOSYNTHESIS;
ALPHA-ISOPROPYLMALATE;
BETA-ISOPROPYLMALATE;
D O I：
10.1016/S0232-4393(11)80257-X
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ;
0830 ;
摘要：
We isolated a mutant from Candida maltosa lacking beta-isopropylmalate dehydrogenase (leu2), which was able to excrete alpha- and beta-isopropylmalate into the culture medium. The effects of incubation time, L-leucine concentration, and carbon and nitrogen sources on beta-isopropylmalate production were examined. In the presence of 20 g glucose/1, 5 g NH4H2PO4/1 and 75 mg L-leucine/1 in a minimal salt medium C. maltosa G587 produced about 900 mg beta-isopropylmalate/1 during 72 h of growth. A simple procedure to isolate both dicarboxylic acids from the medium is described.
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页码：35 / 39
页数：5
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