BACITRACIN PRODUCTION BY A NEW STRAIN OF BACILLUS-SUBTILIS - EXTRACTION, PURIFICATION, AND CHARACTERIZATION

被引：46

作者：

AZEVEDO, EC

RIOS, EM

FUKUSHIMA, K

CAMPOSTAKAKI, GM

机构：

[1] UNIV FED PERNAMBUCO,MESTRADO IMUMOPATOL PROF KEIZO ASAMI LIKA LAB,BR-50739 RECIFE,PE,BRAZIL

[2] UNIV FED PERNAMBUCO,DEPT ANTIBIOT,BR-50739 RECIFE,PE,BRAZIL

[3] UNIV FED PERNAMBUCO,MESTRADO CIENCIAS FARMACEUT,BR-50739 RECIFE,PE,BRAZIL

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1993年 / 42卷 / 01期

关键词：

BACILLUS-SUBTILIS; BACITRACIN PRODUCTION; MICROCOCCUS-FLAVUS;

D O I：

10.1007/BF02788897

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new strain of Bacillus subtilis C 126 was isolated from sugar cane fermentation and produced an antibiotic that inhibited the growth of Micrococcus flavus. The production of the antibiotic in culture medium followed to extraction with n-butanol, thin layer chromatography, and microbiological tests indicated that a polypeptide antibiotic was produced. The fraction obtained by Sephadex G-25 column and analyzed by HPLC indicated that bacitracin complex was produced.

引用

页码：1 / 9

页数：9

共 31 条

[1] BACITRACIN - METHODS OF PRODUCTION, CONCENTRATION, AND PARTIAL PURIFICATION, WITH A SUMMARY OF THE CHEMICAL PROPERTIES OF CRUDE BACITRACIN [J].