Antibiotic-resistance cassettes for Bacillus subtilis

被引：523

作者：

GueroutFleury, AM ^{[1
]}

Shazand, K ^{[1
]}

Frandsen, N ^{[1
]}

Stragier, P ^{[1
]}

机构：

[1] INST BIOL PHYSICOCHIM, F-75005 PARIS, FRANCE

来源：

GENE | 1995年 / 167卷 / 1-2期

关键词：

erythromycin; kanamycin; tetracycline; spectinomycin; chromosomal disruption;

D O I：

10.1016/0378-1119(95)00652-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 [遗传学]; 090102 [作物遗传育种];

摘要：

The genes encoding resistance to four different antibiotics (erythromycin, kanamycin, tetracycline and spectinomycin) were cloned in the polylinker of various Escherichia coli plasmid vectors. These cassettes can be inserted into cloned Bacillus subtilis (Bs) genes and used to create tagged chromosomal disruptions after recombination into Bs and selection in the presence of the appropriate antibiotic.

引用

页码：335 / 336

页数：2

共 8 条

[1]

A GENE FOR A NEW LIPOPROTEIN IN THE DAPA-PURC INTERVAL OF THE ESCHERICHIA-COLI CHROMOSOME [J].