IMMUNOCHEMICAL IDENTIFICATION AND TRANSLOCATION OF PROTEIN-KINASE-C-ZETA IN HUMAN NEUTROPHILS

被引:64
作者
DANG, PMC
HAKIM, J
PERIANIN, A
机构
[1] CHU XAVIER BICHAT,INSERM,U294,HEMATOL LAB,F-75018 PARIS,FRANCE
[2] HOP COCHIN,DEPT PHARMACOL,CNRS,URA 1534,F-75674 PARIS,FRANCE
关键词
PROTEIN KINASE C; ISOTYPE; NEUTROPHIL; PHORBOL ESTER;
D O I
10.1016/0014-5793(94)00700-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Western blots of human polymorphonuclear leukocyte (PMN) extracts were immunostained with antibodies specific for various protein kinase C (PKC) isoforms. Two bands corresponding to PKC type zeta with apparent molecular masses of 81 kDa and 76 kDa were identified in the cytosolic fraction of resting cells, in addition to PKC types alpha and beta. PKC zeta was apparently abundant, like PKC beta, whereas PKC delta, -epsilon, and -gamma were not detectable. Following short stimulation (5 min) of PMN with phorbol-12-myristate-13-acetate (1 mu g/ml), physical translocation of PKC zeta from the cytosol to the plasma membrane fraction occurred, although this isoform does not bind phorbol esters. These data show that, in addition to the two calcium-dependent isoenzymes alpha and beta, human PMN express a calcium-independent isoenzyme zeta which translocates in stimulated cells, suggesting a role in the regulation of antibacterial activities.
引用
收藏
页码:338 / 342
页数:5
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