GENETIC KNOCKOUTS IN MICE - AN UPDATE

被引：19

作者：

SHASTRY, BS

机构：

[1] Eye Research Institute, Oakland University, Rochester, 48309-4401, Michigan

来源：

EXPERIENTIA | 1995年 / 51卷 / 11期

关键词：

GENE DISRUPTION; GENE TARGETING; HOMOLOGOUS RECOMBINATION; PHENOTYPE; REDUNDANCY; STEM CELLS;

D O I：

10.1007/BF01946911

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Gene disruption technology in mammals, by homologous recombination in embryonic stem cells, is a powerful method to manipulate the mouse germ line. In the past decade it has produced a wealth of knowledge concerning neuronal development, neurodegenerative disorders and the roles of oncogenes, Hox genes and growth factors during development. A surprising variety of genes, however, have given unexpected and disappointing results. A gene/function redundancy theory proposed by many investigators to explain the unexpected results has been supported in certain cases by the generation of double knockout mice. Modification of the basic technology now allows the investigators to carry out a variety of manipulations including conditional or tissue-specific knockouts. This may provide a better opportunity in the future for the gene therapy approach to correct the genetic disorder.

引用

页码：1028 / 1039

页数：12

共 175 条

[1] REDUCED HIPPOCAMPAL LONG-TERM POTENTIATION AND CONTEXT-SPECIFIC DEFICIT IN ASSOCIATIVE LEARNING IN MGLUR1 MUTANT MICE [J].