ISOLATION AND STRUCTURE OF DIAPAUSE HORMONE OF THE SILKWORM, BOMBYX-MORI

被引：198

作者：

IMAI, K

KONNO, T

NAKAZAWA, Y

KOMIYA, T

ISOBE, M

KOGA, K

GOTO, T

YAGINUMA, T

SAKAKIBARA, K

HASEGAWA, K

YAMASHITA, O

机构：

[1] NAGOYA UNIV,SCH AGR,SERICULTURAL SCI LAB,CHIKUSA KU,NAGOYA 46401,JAPAN

[2] NAGOYA UNIV,SCH AGR,ORGAN CHEM LAB,CHIKUSA KU,NAGOYA 46401,JAPAN

[3] MIE UNIV,FAC BIORESOURCES,CHEMURGY LAB,TSU,MIE 514,JAPAN

来源：

PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES | 1991年 / 67卷 / 06期

关键词：

DIAPAUSE HORMONE; PEPTIDE; AMINO ACID SEQUENCE; SUBESOPHAGEAL GANGLION; BOMBYX-MORI;

D O I：

10.2183/pjab.67.98

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The embryonic diapause of the silkworm, Bombyx mori is induced by a neuropeptide hormone, diapause hormone, which is secreted from the suboesophageal ganglion. About 55,000 complexes of the suboesophageal ganglion and the first thoracic ganglion were dissected out from day 1 pupae of silkworms. The hormone was extracted with hot water and purified by a reverse-phase high performance liquid chromatography on a TSK gel octadecyl-4PW column. The second chromatography gave a single symmetric peak and the isolated hormone induced diapause in 20% eggs at a dose of 1.5 ng/pupa. Diapause hormone has 24 amino acid residues and a molecular weight of 2645. The synthesized peptide with a carboxyl terminal as amide form exhibited the same behavior on HPLC and similar activity as the natural hormone.

引用

页码：98 / 101

页数：4

共 17 条

[1] HOMOLOGIES BETWEEN THE AMINO-ACID-SEQUENCES OF SOME VERTEBRATE PEPTIDE-HORMONES AND PEPTIDES ISOLATED FROM INVERTEBRATE SOURCES [J].