INABILITY OF PSEUDOMONAS-STUTZERI DENITRIFICATION MUTANTS WITH THE PHENOTYPE OF PSEUDOMONAS-AERUGINOSA TO GROW IN NITROUS-OXIDE

Pseudomonas aeruginosa PAO1 reduced nitrous oxide to dinitrogen but did not grow anaerobically in nitrous oxide. Two transposon insertion Nos- mutants of Pseudomonas stutzeri exhibited the P. aeruginosa phenotype. Growth yield studies demonstrated that nitrous oxide produced in vivo was productively respired, but nitrous oxide supplied exogenously was not. The defect may be in electron transport or in nitrous oxide uptake.