CLONING AND EXPRESSION OF A CDNA-ENCODING THE TRANSPORTER OF TAURINE AND BETA-ALANINE IN MOUSE-BRAIN

被引：251

作者：

LIU, QR ^{[1
]}

LOPEZCORCUERA, B ^{[1
]}

NELSON, H ^{[1
]}

MANDIYAN, S ^{[1
]}

NELSON, N ^{[1
]}

机构：

[1] ROCHE INST MOLEC BIOL,ROCHE RES CTR,NUTLEY,NJ 07110

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 24期

关键词：

NEUROTRANSMITTERS; MEMBRANE PROTEIN; MOLECULAR CLONING;

D O I：

10.1073/pnas.89.24.12145

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A taurine/beta-alanine transporter was cloned from a mouse brain cDNA library by screening with a partial cDNA probe of the glycine transporter at low stringency. The deduced amino acid sequence predicts 590 amino acids with typical characteristics of the sodium-dependent neurotransmitter transporters such as sequence homology and membrane topography. However, the calculated isoelectric point of the taurine/beta-alanine transporter is more acidic (pI = 5.98) than those (pI > 8.0) of other cloned neurotransmitter transporters. Xenopus oocytes injected with cRNA of the cloned transporter expressed uptake activities with K(m) = 4.5 muM for taurine and K(m) = 56 muM for beta-alanine. Northern hybridization showed a single transcript of 7.5 kilobases that was highly enriched in kidney and distributed evenly in various parts of the brain. In situ hybridization showed the mRNA of the taurine/beta-alanine transporter to be localized in the corpus callosum, striatum, and anterior commisure. Specific localization of the taurine/beta-alanine transporter in mouse brain suggests a potential function for taurine and beta-alanine as neurotransmitters.

引用

页码：12145 / 12149

页数：5

共 39 条

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