CAPILLARY ELECTROPHORETIC EXAMINATION OF UNDERIVATIZED O-LINKED AND N-LINKED OLIGOSACCHARIDE MIXTURES AND IMMUNOGLOBULIN-G ANTIBODY-RELEASED OLIGOSACCHARIDE LIBRARIES

被引：9

作者：

HUGHES, DE

机构：

[1] Pharmaceutical Research Institute, Bristol-Myers Squibb Company, Syracuse, NY 13221-4755

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 657卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00094-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure for the analysis of mixtures of underivatized, cleaved O-linked and N-linked oligosaccharides by capillary electrophoresis has been developed. The species of interest are separated by two berate-based buffer systems with an uncoated capillary. The procedure is applied to hydrazinolysis-released oligosaccharides from mouse, rat, sheep and human IgG protein samples which are examined within 6 minutes. Selectivity with respect to sample variation is demonstrated by analysis of thermally stressed samples.

引用

页码：315 / 326

页数：12

共 15 条

[1]

Chaplin M. F., 1986, CARBOHYDRATE ANAL PR, P37

[2]

GARNER T, 1990, J CHROMATOGR, V515, P373

[3] THE MAPPING BY HIGH-PH ANION-EXCHANGE CHROMATOGRAPHY WITH PULSED AMPEROMETRIC DETECTION AND CAPILLARY ELECTROPHORESIS OF THE CARBOHYDRATE MOIETIES OF HUMAN PLASMA ALPHA(1)-ACID GLYCOPROTEIN [J].