COLORIMETRIC MICROMETHODS FOR GLUTARALDEHYDE DETERMINATION BY MEANS OF PHENOL AND SULFURIC-ACID OR PHENOL AND PERCHLORIC-ACID

被引：36

作者：

BORATYNSKI, J

ZAL, T

机构：

[1] Department of Microbiology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 184卷 / 02期

关键词：

D O I：

10.1016/0003-2697(90)90677-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Aqueous micromolar solutions of glutaraldehyde produce a yellow color when treated with 20% phenol in ethanol followed by concentrated sulfuric acid or with phenol in 70% perchloric acid. The absorbance at 482 or 479 nm, respectively, is linearly related to the glutaraldehyde concentration. Of the two methods developed, the sulfuric acid-phenol assay gives a higher sensitivity and the perchloric acid-phenol assay allows the determination of glutaraldehyde in the presence of sugars and proteins. © 1990.

引用

页码：259 / 262

页数：4

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