DETERMINATION OF 5-METHOXYINDOLES IN PINEAL-GLAND AND PLASMA SAMPLES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION

被引：42

作者：

RAYNAUD, F

PEVET, P

机构：

[1] URA CNRS 1332, Neurobiologie des Fonctions Rythmiques et Saisonnières, Laboratoire de Zoologie, 67000 Strasbourg

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 564卷 / 01期

关键词：

D O I：

10.1016/0378-4347(91)80073-L

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A liquid chromatographic analysis with electrochemical detection of 5-methoxytryptamine, 5-methoxytryptophol, 5-methoxyindoleacetic acid and melatonin is described. Optimal elution conditions were determined by studying several variables: pH, buffer salt, counter ion and organic modifier. Measurement of 5-methoxyindoles in the pineal gland and plasma of hamsters has been performed after extraction. This method is specific and sensitive, and enables detection of 5-methoxyindoles in a pool of two hamster pineal glands. This is also the first time that these three 5-methoxyindoles have been measured simultaneously in plasma.

引用

页码：103 / 113

页数：11

共 14 条

[1]

CHIN JL, 1988, J CHROMATOGR-BIOMED, V428, P206

[2] PINEAL INDOLES - SIGNIFICANCE AND MEASUREMENT [J].

FOLEY, PB ;

CAIRNCROSS, KD ;

FOLDES, A .

NEUROSCIENCE AND BIOBEHAVIORAL REVIEWS, 1986, 10 (03) :273-293

[3] DAY NIGHT RHYTHM OF 5-METHOXYTRYPTAMINE BIOSYNTHESIS IN THE PINEAL-GLAND OF THE GOLDEN-HAMSTER (MESOCRICETUS-AURATUS) [J].

GALZIN, AM ;

EON, MT ;

ESNAUD, H ;

LEE, CR ;

PEVET, P ;

LANGER, SZ .

JOURNAL OF ENDOCRINOLOGY, 1988, 118 (03) :389-397

[4]

HEREGODT P, 1985, J CHROMATOGR, V345, P33

[5] DETERMINATION OF PINEAL MELATONIN BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION - APPLICATION FOR RHYTHM STUDIES AND TISSUE EXPLANTS [J].

HERNANDEZ, G ;

ABREU, P ;

ALONSO, R ;

CALZADILLA, CH .

JOURNAL OF PINEAL RESEARCH, 1990, 8 (01) :11-19

[6]

MCCLINTOCK SA, 1987, PROGR HPLC ELECTROCH, P37

[7] DETERMINATION OF TRYPTOPHAN AND METABOLITES IN RAT-BRAIN AND PINEAL TISSUE BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION [J].

MEFFORD, IN ;

BARCHAS, JD .

JOURNAL OF CHROMATOGRAPHY, 1980, 181 (02) :187-193

[8]

MEFFORD IN, 1983, ENDOCRINOLOGY, V113, P1583

[9]

NAMBOODIRI MAA, 1983, METHODS BIOGENIC AMI, P549

[10] THE EFFECT OF 5-METHOXYTRYPTAMINE ON GOLDEN-HAMSTER GONADS IS NOT A CONSEQUENCE OF ITS ACETYLATION INTO MELATONIN [J].

RAYNAUD, F ;

MIGUEL, JL ;

VIVIENROELS, B ;

MASSONPEVET, M ;

PEVET, P .

JOURNAL OF ENDOCRINOLOGY, 1989, 121 (03) :507-512

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