EVIDENCE FOR THE ROLE OF SUBGENOMIC RNA SPECIES IN THE PRODUCTION OF HELENIUM VIRUS-S COAT PROTEIN DURING INVITRO TRANSLATION

The genomic RNA (7.4 kb) and two subgenomic RNAs of 3.3 kb and 1.5 kb were detected in RNA from purified virus particles of Helenium virus S (HelVS) by Northern hybridization with a clone specific to the viral coat protein gene. In rabbit reticulocyte lysate, the 7.4 kb RNA directed the synthesis of two polypeptides of Mr 190,000 (190 kDa) and 160 kDa. The 1.5 kb RNA directed the synthesis of two products, both at a Mr. of approximately 32 kDa which were immunoprecipitated with antiserum to HelVS virus particles. Virus particle lengths suggest that the subgenomic RNAs may be encapsidated in 180 to 320 nm particles. © 1990.