ISOLATION AND CHARACTERIZATION OF A DIURETIC PEPTIDE FROM ACHETA-DOMESTICUS - EVIDENCE FOR A FAMILY OF INSECT DIURETIC PEPTIDES

被引:80
作者
KAY, I [1 ]
COAST, GM [1 ]
CUSINATO, O [1 ]
WHEELER, CH [1 ]
TOTTY, NF [1 ]
GOLDSWORTHY, GJ [1 ]
机构
[1] LUDWIG INST CANC RES,LONDON W1P 8BT,ENGLAND
来源
BIOLOGICAL CHEMISTRY HOPPE-SEYLER | 1991年 / 372卷 / 07期
关键词
DIURETIC PEPTIDE; ACHETA-DOMESTICUS; CAMP; FLUID SECRETION; PEPTIDE HORMONE FAMILY;
D O I
10.1515/bchm3.1991.372.2.505
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A diuretic peptide (Acheta-DP) has been isolated from extracts of whole heads of the house cricket, Acheta domesticus. The native peptide increases both cyclic AMP production and the rate of fluid secretion by isolated Malpighian tubules in vitro to an extent comparable with those responses obtained with supra-maximal amounts of crude extracts of corpora cardiaca. The primary structure of Acheta-DP was established as a 46-residue amidated peptide: TGAQSLSIVAPLDVLRQRLMNELN-RRRMRELQGSRIQQNRQLLTSI-NH2. Acheta-DP has 41% sequence identity with a diuretic peptide isolated from Manduca sexta, providing direct evidence for the presence of a family of diuretic peptides in insects.
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页码:505 / 512
页数:8
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