CHLORPHENIRAMINE BINDING TO HUMAN SERUM-ALBUMIN BY FLUORESCENCE QUENCHING MEASUREMENTS

被引：24

作者：

GONZALEZJIMENEZ, J

FRUTOS, G

CAYRE, I

CORTIJO, M

机构：

[1] Departamento de Química Física Farmaceútica, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Ciudad Universitaria

来源：

BIOCHIMIE | 1991年 / 73卷 / 05期

关键词：

CHLORPHENIRAMINE; ANTIHISTAMINIC; ALBUMIN; FLUORESCENCE; QUENCHING; BINDING;

D O I：

10.1016/0300-9084(91)90022-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The binding of chlorpheniramine to human serum albumin has been studied by fluorescence quenching, as a function of temperature; the experimental data could only be fitted to the Stern-Volmer modified equation. A statistical analysis of the results was performed in order to determine the significance of the constants calculated by this equation, as well as their thermodynamic parameters. The chlorpheniramine binding to human serum albumin accounts for almost half of the binding of this antihistaminic agent to human plasma proteins.

引用

页码：551 / 556

页数：6

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