CHARACTERIZATION OF A POLLEN-EXPRESSED GENE ENCODING A PUTATIVE PECTIN ESTERASE OF PETUNIA-INFLATA

被引：82

作者：

MU, JH ^{[1
]}

STAINS, JP ^{[1
]}

KAO, TH ^{[1
]}

机构：

[1] PENN STATE UNIV,DEPT BIOCHEM & MOLEC BIOL,UNIVERSITY PK,PA 16802

来源：

PLANT MOLECULAR BIOLOGY | 1994年 / 25卷 / 03期

关键词：

PECTIN ESTERASE; PETUNIA INFLATA; POLLEN; POLLEN TUBES;

D O I：

10.1007/BF00043881

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

From a pollen tube cDNA library of Petunia inflata, we isolated cDNA clones encoding a protein, PPE1, which exhibits sequence similarity with plant, bacterial, and fungal pectin esterases. Genomic clones containing the PPE1 gene were isolated using cDNA for PPE1 as a probe, and comparison of the cDNA and genomic sequences revealed the presence of a single intron in the PPE1 gene. During pollen development, PPE1 mRNA was first detected in anthers containing uninucleate microspores; it reached the highest level in mature pollen and persisted at a high level in in vitro germinated pollen tubes. The observed expression pattern of the PPE1 gene suggests that its product may play a role in pollen germination and/or tube growth.

引用

页码：539 / 544

页数：6

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